一、課程簡介:

1. Web
2. I.Enzymology of recombinant DNA-Restriction enzymes
3. II.Enzymology of recombinant DNA-Enzymes in DNA engineering
4. Isolation of nucleic acids, blotting and DNA sequencing
5. Plasmids and cloning strategies
6. Bacteriophage, cosmid, YAC
7. (Mid-term examination)
8. Primer design, PCR strategies, RACE
9. Hybridization probes and screening
10. Analyses of gene regulation
11. Construction of cDNA and genomic DNA libraries
12. Expression systems and purification
13. Transgenic animal and gene targeting
14. whole-mount in situ hybridization and other techniques


二、先修課程:

三、參考書目:1.Greene, J. J. and Rao, V. B. 1998 Recombinant DNA Principles and Methodologies.
2.Old, R. W. and Primrose, S. B. 2002 Principles of Gene Manipulation.